Indexed In Scopus
  Scopus ID: 21100926589

Optimization of Caffeic Acid and its Antibacterial Activity Using Carica papaya Seed Extract Nanoparticles (CPNPs)

Gumma Jayasri, Akhil Arava, Sandeep Sirohi, Avinash Singh, D. Appala Naidu and Meena Vangalpati

Abstract

The seeds of the papaya (Carica papaya) have long been used for their therapeutic benefits, including possible antibacterial activity. In this study, we used response surface methodology (RSM) to optimize the extraction process and assess the antibacterial activity of C. papaya seed nanoparticles (CPNPs) ethanol extract against selected bacterial strains. The papaya seeds were dried and powdered. CPNPs were synthesized using C. papaya extract through a green and sustainable approach. The biosynthesized NPs were characterized for their size, morphology, and composition by scanning electron microscope (SEM) analysis. Using the agar well diffusion method, the antibacterial activity was determined against typical bacterial strains, which include Salmonella and Staphylococcus aureus. To enhance the extract’s antibacterial activity, other extraction process parameters including the solid-to-liquid ratio, ethanol concentration, and extraction duration were further adjusted using RSM. Box-Behnken design (BDD) was used to create the experimental design matrix. The purpose of this study was to optimize the extraction procedure using Fourier transform infrared spectroscopy (FTIR) and RSM and to investigate the antibacterial qualities of CPNPs ethanol extract.

Published on: March 14, 2024
doi: 10.17756/nwj.2024-s1-023
Citation: Jayasri G, Arava A, Sirohi S, Singh A, Naidu DA, et al. 2024. Optimization of Caffeic Acid and its Antibacterial Activity Using Carica papaya Seed Extract Nanoparticles (CPNPs). NanoWorld J 10(S1): S127-S131.

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