Green nanoparticle synthesis is becoming more popular these days due to its low toxicity as compared to chemical methods. To investigate the structure and morphology of nanoparticles, a zinc nitrate solution is used for Zinc nanoparticle (Zn-NP) synthesis of P. nigrum. This aqueous of seed was utilized in the biogenesis of the Zinc nanoparticles to assess its potential as an effective antimicrobial agent. The surface plasma resonance band at 274 nm, revealed the production of Zn-NPs. Fourier transform infrared detected the presence of functional groups similar to the spectra of Cubebin, the secondary metabolite of P. nigrum. Energy- dispersive X-ray analysis showed an elemental make-up of 89.4% Zinc content in the nanoparticle. Cubebin in seed extract may be the eco-friendly green factor responsible in an aqueous medium as a reducing and capping agent. This green biogenic method was quick and easy to implement, and it could quickly replace chemical production. The bactericidal activity of Zn-NP of P. nigrum was pronounced against both gram-positive and gram-negative bacterial isolates. The research demonstrated that the secondary metabolites of the dried seed extract can be a suitable capping agent in the synthesis of the nanoparticles as well as responsible for antimicrobial activities.

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