Abstract
Titanium dioxide nanoparticles (TiO2NPs) are supposed to be the most widely engineered nanoparticles (NPs) that are used in the world. The study evaluated the toxicity of TiO2NPs (12 – 64 nm) in male mice by intraperitoneal injection at five doses of either control (0), 2.5, 5.0, 10.0, and 20.0 mg/kg of body weight for 28 d. Hematological changes and oxidative stress indicators like Catalase (CAT),
Glutathione-S-transferase (GST), Glutathione peroxidase (GPx), Glutathione reductase (GR), Lipid peroxidation (LPO), Glutathione (GSH) content as well as Carbonyl protein (CP) were explored. Present results showed significant differences (P < 0.05) in all hematological measurements at the end of the study for treated groups, respect to their control. TiO2NPs caused dose-dependent oxidative stress in lung, liver, spleen, kidney, heart, and muscle samples of the treated animals, concerning the control group. Noticeable important increases (P < 0.05) of malondialdehyde (MDA) levels were represented in all treatments of samples as following: heart > muscle > spleen > liver > kidney > lung, with respect to their controls. Also, noticeable important increases were recorded for GPx activity and CP level. However, significant declines were noted in CAT, and GST activities, and GSH content in treated animals, concerning control. Moreover, excess of such CP, MDA, and other biomarker alterations may provide a fingerprint of potential toxic effects of TiO2NPs during long-term exposure scenario. Thus, extensive application of such NPs raises the consideration about biosafety.
doi: 10.17756/nwj.2021-088
Citation: Abdel-Halim KY, Osman SR, Abuzeid MAF, Khozimy AM. 2021. Hematological Changes and Oxidative Stress Induction of Titanium Dioxide Nanoparticles in Male Mice after Intraperitoneal Injection of Different Doses for 28 Days: Study of Organ’s Responsibility. NanoWorld J 7(1): 22-32.
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